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Image Search Results
Journal: Nucleic Acids Research
Article Title: A conserved 3′UTR short motif regulates gene expression in vertebrates
doi: 10.1093/nar/gkaf1340
Figure Lengend Snippet: Microinjection of DplUSE RNA causes a decrease in GFP expression in transgenic GFP-DplUSE animals. ( A ) Representative image of a 5 dpf GFP-DplUSE transgenic line. ( B ) Confocal images of transgenic GFP-DplUSE zebrafish, stained with the nuclear marker DAPI, showing GFP expression in the notochord and muscle (scale bar = 10 μm). ( C ) Representative image of GFP expression in transgenic GFP-DplUSE zebrafish embryos microinjected with DplUSE RNA or DplUSEmt RNA at 5 dpf. Images were acquired using Leica M205. ( D ) Quantification of GFP expression in notochord ( n = 75 Non Injected (NI); n = 64 DplUSE; n = 54 DplUSEmt) and ( E ) in muscle ( n = 70 NI; n = 53 DplUSE; n = 54 DplUSEmt). ( F ) Quantification of the percentage of transgenic GFP-DplUSE embryos that showed a patchy expression of GFP in the notochord (36.5%, n = 73 NI; 73%, n = 69 DplUSE; 50%, n = 59 DplUSEmt). The dashed lines represent the regions where GFP was quantified. Statistical significance was determined by χ2 test with Fisher correction or by two-tailed unpaired t-test. *** P < .001; ** P < .01; * P < .05; ns P > .05. (scale bar = 100 μm).
Article Snippet: Embryos were raised until they reached the 4 dpf larval stage, fixed, and stained with
Techniques: Microinjection, Expressing, Transgenic Assay, Staining, Marker, Injection, Two Tailed Test
Journal: Oncology Letters
Article Title: Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation
doi: 10.3892/ol.2018.8019
Figure Lengend Snippet: Identification of CTCs in HCC patients using iFISH platform. CK: Green, CEP8: Orange, DAPI: Blue, CD45: Red. (A) CK+/CD45-/DAPI+/CEP8=2; (B) CK+/CD45-/DAPI+/CEP8>2; (C) CK-/CD45+/DAPI+/CEP8>2. (D) CTC cluster. (E) CK-/CD45+/DAPI+/CEP8=2, WBC. iFISH, immunostaining-fluorescence in situ hybridization; CTCs, circulating tumor cells; HCC, hepatocellular carcinoma; CK, cytokeratin; CEP8, centromere probe 8; CD, cluster of differentiation; WBC, white blood cell.
Article Snippet: Immunofluorescence reagent (cat no. 7900001;
Techniques: Immunostaining, Fluorescence, In Situ Hybridization